Quartz-Seq protocol文章訂正と追記

Quartz-Seqのプロトコルに一部誤字があったので訂正しました。
またよく質問を受けるPCRプライマーの修飾の種類とメーカー情報に関して追記しました。
訂正版は、これまでと同じく所属ラボRIKEN ACCC BiTからダウンロードできます。
http://bit.accc.riken.jp/protocols/

• Whole-transcript amplification for single-cell Quartz-Seq (PDF)
• Whole-transcript amplification for cell-population Quartz-Seq (Cell Direct Version)
• Whole-transcript amplification for cell-population Quartz-Seq (Purified RNA Version)

追記部分以下の通り。
<PCR primer information>
I used NH2(C6) amination as 5' modification for suppression PCR primer. The amination block the ligation between WTA adaptor and Y-shaped sequence adaptor. Aminated oligo (from some maker) became small size ladder DNA in PCR step. Non-aminated oligo never become ladder DNA. The ladder DNA may affect sequence reads. I don't know the mechanism of ladder DNA synthesis from aminated oligo DNA. On the other hand, Sigma Aldrich oligo and Takara-clontech oligo become few amount of ladder DNA. Therefore, we recommend Sigma Aldrich oligo or Takara-clontech. If you use Nextera system or Nextera XT system for Illumina sequence library preparation using amplified cDNA, you do not need 5' amination for suppression PCR primer.

Tagging primerとPCR primerをまちがって使っちゃった人いたらごめんなさい。